Fig. 3.

MiR-29b-3p targets the 3’UTR of TRAF5. a – The predicted miR-29b-3p-binding sites located in the 3’UTR of TRAF5. b – The effect of miR-29b-3p on luciferase activity was detected using the dual-luciferase reporter assay. The pmirGLO reporter vector containing the WT or MUT TRAF5 was co-transfected with miR-29b-3p or miR-NC into AC16 cells for 48 h. c and d – The mRNA and protein expressions of TRAF5 in AC16 cells transfected with miR-29b-3p or miR-NC and then subjected to hypoxia for 24 h were determined using quantitative real time PCR and western blot analysis. Each experiment was repeated three times. ***p < 0.001