Fig. 1.

Canine intestinal 3D organoids (enteroids and colonoids) from healthy and inflammatory bowel disease (IBD) dogs. Representative phase contrast images at × 40 magnification of fully differentiated 5–7-day-old duodenal and ileal enteroids and colonoids from healthy dogs and dogs with IBD, as well as gastrointestinal stromal tumor (GIST) and colorectal adenocarcinoma tumor (tumors at × 20 magnification). The structures of enteroids and colonoids from healthy dogs and dogs with IBD appeared comparable. Representative photomicrographs of at least n = 15 (healthy dogs), n = 7 (IBD dogs), and n = 4 (tumors) images of enteroids/colonoids are shown for each location as indicated