Fig. 8.

Forskolin induces swelling of canine enteroids in a time-dependent manner, indicating presence of functional CFTR. Enteroids were passaged and seeded in Matrigel into 24-well plates. After 2 days, enteroids were incubated in media containing vehicle control (DMSO) or 10 μM forskolin. Representative images of enteroids were taken after 0, 1, 4, and 24 h, at × 5 magnification. Graph of mean area of enteroids (15–25 per field) from n = 12 fields per condition as determined by ImageJ (mean + SEM; p < 0.05 vs. control for each time)