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. 2019 Apr 5;10:711. doi: 10.3389/fimmu.2019.00711

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Copyright © 2019 Wimmer, Tietz, Nishihara, Deutsch, Sallusto, Gosselet, Lyck, Muller, Lassmann and Engelhardt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PECAM-1^−/− pMBMECs display an intact junctional architecture. Immunofluorescent stainings of WT and PECAM-1^−/− pMBMEC monolayers were performed for PECAM-1 (A), VE-cadherin (B), claudin-5 (C), JAM-A (C), occludin (C), and ZO-1 (D). Additionally, the fluorescent signal of VE-CadGFP-expressing pMBMECs is shown (B). Also, stainings for mouse and rabbit isotype controls are presented (E). Cell nuclei were stained with DAPI. Merged grayscale pictures of nucleic signals and the specific antibody stainings are shown. Scale bars, 50 μm.