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. 2019 Apr 5;10:711. doi: 10.3389/fimmu.2019.00711

Figure 7.

Figure 7

Lack of endothelial PECAM-1 does not affect expression levels of ICAM-1 on pMBMECs. (A) WT and PECAM-1−/− pMBMEC monolayers were treated for 16 h either with IL-1β (20 ng/ml) or left unstimulated. Western blots of cell lysates were incubated with anti-ICAM-1 rabbit serum and rabbit anti-GAPDH antibody. One representative experiment out of 4 is shown. (B) Quantified ICAM-1 signals were normalized to the reference protein GAPDH. For each experiment, normalized signals were further related to the unstimulated WT sample. Each individual data point is shown and shapes correlate to the respective independent experiments (n = 4). Reported statistics result from unpaired, two-tailed Student's t-test. Test statistics: p = 0.348, t(6) = 1.018, d = 0.831. Error bar, ± SD; ns, not significant. (C) Untreated and IL-1β-stimulated VE-CadGFP endothelial monolayers stained for ICAM-1 expression (rat hybridoma supernatant 25ZC7). Blue, cell nuclei stained with DAPI; Scale bar, 60 μm.