Table 1.
Values for retinal vascular, thickness, and functional parameters in RA and OIR mice
| 1A. Vascular parameters in OIR and RA mice per age | ||||||||
|---|---|---|---|---|---|---|---|---|
| Age | OIR (mean ± SD, n) | RA (mean ± SD, n) | †P value | |||||
| P20 | RAA | 71.11 | 10.15 | 9 | 67.75 | 8.51 | 8 | 0.4229 |
| RVW | 62.42 | 5.15 | 9 | 54.49 | 2.46 | 8 | 0.0044 | |
| RAT | 1.16 | 0.07 | 9 | 1.00 | 0.00 | 8 | 0.0001 | |
| ‡Weight | 8.44 | 0.73 | 9 | 8.88 | 0.83 | 8 | 0.4600 | |
| P40 | RAA | 55.33 | 5.66 | 9 | 60.50 | 9.03 | 10 | 0.1963 |
| RVW | 50.34 | 7.19 | 9 | 48.48 | 5.19 | 10 | 0.4524 | |
| RAT | 1.09 | 0.05 | 9 | 1.00 | 0.00 | 10 | 0.0001 | |
| ‡Weight | 17.44 | 1.81 | 9 | 19.80 | 1.03 | 10 | 0.0001 | |
| 1B. Retinal thickness in RA and OIR mice per age | ||||||||
| Age | OIR (mean ± SD, n) | RA (mean ± SD, n) | †P value | |||||
| P20 vascular | TRT | 175.44 | 8.81 | 9 | 191.93 | 6.69 | 8 | < 0.0001 |
| ORT | 93.33 | 6.79 | 9 | 93.64 | 3.15 | 8 | 0.8853 | |
| IRT | 82.11 | 4.15 | 9 | 98.28 | 7.32 | 8 | < 0.0001 | |
| P20 avascular | TRT | 170.27 | 11.53 | 9 | n/a | |||
| ORT | 90.51 | 2.76 | 9 | |||||
| IRT | 79.78 | 10.23 | 9 | |||||
| P40 vascular | TRT | 159.63 | 6.59 | 9 | 177.69 | 6.11 | 10 | < 0.0001 |
| ORT | 92.50 | 4.02 | 9 | 92.33 | 1.65 | 10 | 0.9320 | |
| IRT | 67.16 | 5.79 | 9 | 85.34 | 5.80 | 10 | < 0.0001 | |
| 1C. ERG parameters in RA and OIR per age | ||||||||
| Age | ERG | OIR (mean ± SD) log10cd sec/m2 | RA (mean ± SD) log10cd sec/m2 | |||||
| 0.3 | 1.8 | 3.3 | 0.3 | 1.8 | 3.3 | |||
| P20 | a-wave | −7.08 ± 6.45 | −13.34 ± 6.21 | −29.48 ± 29.05 | −19.06 ± 22.95 | −7.40 ± 6.62 | −22.06 ± 15.82 | |
| b-wave | 24.76 ± 11.88 | 29.20 ± 7.08 | 67.01 ± 36.13 | 28.83 ± 20.36 | 26.58 ± 11.02 | 75.85 ± 39.38 | ||
| a-wave peak time | 45.29 ± 39.84 | 44.58 ± 30.73 | 35.62 ± 13.25 | 60.78 ± 36.25 | 34.93 ± 28.96 | 45.60 ± 36.00 | ||
| b-wave peak time | 67.67 ± 45.13 | 80.09 ± 55.18 | 75.87 ± 13.99 | 45.08 ± 36.63 | 78.20 ± 29.45 | 68.50 ± 6.22 | ||
| P40 | a-wave | −22.33 ± 10.30 | −17.64 ± 10.18 | −25.48 ± 15.75 | −22.33 ± 10.30 | −10.64 ± 9.66 | −23.66 ± 18.73 | |
| b-wave | 27.94 ± 16.14 | 25.00 ± 6.38 | 49.68 ± 8.98 | 27.94 ± 16.14 | 33.40 ± 21.99 | 72.45 ± 17.83 | ||
| a-wave peak time | 66.33 ± 37.02 | 58.44 ± 37.40 | 58.02 ± 39.20 | 66.33 ± 37.02 | 47.82 ± 40.61 | 71.40 ± 39.45 | ||
| b-wave peak time | 53.38 ± 57.30 | 66.82 ± 56.74 | 64.69 ± 7.06* | 53.38 ± 57.30 | 59.48 ± 40.19 | 57.46 ± 6.00* | ||
P represents postnatal day age; RA represents mice with room air; OIR represents mice with oxygen-induced retinopathy; SD represents standard deviation
†P value refers to P value between RA and OIR mice for each postnatal day age P20 or P40
‡Weight refers to body weight of each mouse at imaging on P20 or P40, presented in grams
Retinal thickness is presented in µm; n/a—not applicable: there were no avascular areas in RA room air mice at P20, and no avascular capillary areas in both OIR and RA mice at P40
ERG represents electroretinogram values; time to a standards for a-wave peak time; time to b standards for b-wave peak time. *Represents a P value of P = 0.03 for b-wave peak. Time at high intensities (3.3 cd sec/m2). All other P values for ERG parameters not stated were not significant, P > 0.05