Table 1.
Primer sets used for PCR amplification and sequencing
| Target gene | Oligonucleotide sequence (5′–3′) | Specificity | Tm (°C) | Size (bp) | References |
|---|---|---|---|---|---|
| gltA | F-CGAACTTACCGCTATTAGAATGa | Rickettsia genus-specific | 54 | 581 | [30] |
| R-CTTTAAGAGCGATAGCTTCAAGa | |||||
| OmpA | F-TGCAGGGCTTAGATATTCGGCa | Spotted fever group-specific | 48 | 258 | This study |
| R-AAGCTGTTGGTAAAGGAGCAa | |||||
| OmpB | F-GGACCTGAAGCTGGAGCAATa | Rickettsia genus-specific | 48 | 776 | This study |
| R-CTGTCAGGCTGGCTGATGAAa | |||||
| bioB | F-ATGTTCGGGCTTCCGGTATG | R. felis-/ R. asembonensis-specific | 60 | 120 | [31] |
| R-CCGATTCAGCAGGTTCTTCAA | |||||
| Probe 6FAM-GCTGCGGCGGTATTTTAGGAATGGG-TAMRA | |||||
| RfelB | F-TAATTTTAACGGAACAGACGGT | R. felis-specific | 60 | 97 | [32] |
| R-GCCTAAACTTCCTGTAACATTAAAG | |||||
| Probe FAM-TGCTGCTGGTGGCGGTGCTA-BHQ |
aPrimer used for PCR and sequencing
Abbreviations: Tm, annealing temperature; size, amplicon product size, F, forward primer; R, reverse primer