Table 2.

Quantification of secreted HtrA in a time course (strain 81-176)

Time (h)	HtrA concentration (µg/mL)	Molecules of HtrA per mL (× 108)	Bacteria (× 109 CFU/mL)	Molecules HtrA secreted per CFU	Molecules HtrA secreted per CFU (with Caco-2 cells)
t = 2	0.5009 ± 0.1699	59,140 ± 20,070	1.7 ± 0.08	5483 ± 1,246	9640 ± 1078
t = 4	0.6604 ± 0.1623	77,980 ± 19,170	3.0 ± 0.26	3466 ± 814	5279 ± 1655
t = 8	0.8654 ± 0.2006	102,200 ± 23,690	5.1 ± 0.48	2151 ± 562	3263 ± 317

Values were calculated for each experiment separately and then the mean of these values was calculated. The shown error values for the first three columns are SEM and based on the final mean values. The errors for the fourth and fifth column were calculated as described in the “ Materials and methods” section