Fig. 4.

ELISA demonstrating box A binding to RAGE. Soluble RAGE-Fc chimera (0.25 nM) was incubated with an excess amount of box A (10 μM) at room temperature for 30 min. The mixture was then added to HMGB1 coated plate (60 nM) and incubated for 100 min at room temperature. After washing the plates, anti-human HRP antibody was added and incubated for 90 min at room temperature. Optical density (OD) at 450 nm was measured. Ctrl = No addition of RAGE-Fc. *: P < 0.0001