Fig. 5.

The surface topographical features of cells on scaffold imaged by scanning electron microscopy (SEM). a SEM image of β-TCP/chitosan scaffold. b After 14 days of incubation in osteogenic medium, the cells contacted to each other and spread on the surface of the scaffold. Moreover, spherical and polygonal adhesion on the surface and around the pores of the scaffold were observed (arrow). c On day 28, large patches and crumby structure of adhesions were seen on the surface and around the pores of the scaffolds, which was increased significantly compared to 14 days prior (arrow)