Table 1.
List of primers used for RT-qPCR analysis
| Gene ID | Accession No. | Gene Name | Primer Sequence (5′-3′) | Amplicon Length (bp) | Amplification efficiency |
|---|---|---|---|---|---|
| PYT16215 | XM_021518643.1 | RS23 | F:TTACACGAATATCCGCCATCA | 100 | 1.05 |
| R:GTAATCGTTATCGTGCCACTT | |||||
| PYT23375 | XM_021500266.1 | EF1A | F:GCGGTGGTATTGACAAGAGA | 113 | 1.04 |
| R:GTTCACGTTCAGCCTTCAGT | |||||
| PYT15332 | XM_021490269.1 | NDUS4 | F:TGTGAGAAGCTGGTGTGCTA | 108 | 1.00 |
| R:TGTGTCTTCCGTCCATTCTAT | |||||
| PYT08134 | XM_021506528.1 | SELR1 | F:AAGGCTGGATCAGCGTACTT | 97 | 1.04 |
| R:CCGTTCTCACACGCCTTAAT | |||||
| PYT12324 | XM_021496512.1 | EIF3F | F:TGCTTCGCTGTGCCTCATAA | 105 | 1.04 |
| R:TGACTTCAGCTGCATTGACTT | |||||
| PYT24193 | XM_021485429.1 | OLA1 | F:AAGGTCAAGGTCTCGGCAAT | 100 | 1.02 |
| R:CACGTGGACAATCTCTTCGT | |||||
| PYT04221 | XM_021511578.1 | ACT | F:GACAGCTACGTAGGAGATGA | 112 | 1.02 |
| R:TGATGCCAGATCTTCTCCATA | |||||
| PYT19618 | XM_021517608.1 | CYTC | F:TGTATGTGACAGTCCTTGGTT | 126 | 1.01 |
| R:GTGTGGCATTGAGCACACTT | |||||
| PYT19918 | XM_021485125.1 | HEL | F:CAGGAAGCAGTGGACTTACA | 100 | 1.02 |
| R:TCATAACGGTCACCGTAAGAA | |||||
| PYT19808 | XM_021522100.1 | EF1B | F:TAGGCCAGTATGGACCTTCA | 105 | 1.05 |
| R:TCTTCTTCCTCTTCAGAGTCT | |||||
| PYT21544 | XM_021497981.1 | GAPDH | F:GTGTACATGCTGAAGTACGAT | 115 | 1.04 |
| R:CGCTCCATGAAGACAGAGAT | |||||
| PYT03203 | XM_021484929.1 | RPL16 | F:GTTATCTTCAGTACGCTCACA | 103 | 1.00 |
| R:ACGCCATGGATATTCTATCCT |