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. 2019 Apr 5;10:675. doi: 10.3389/fimmu.2019.00675

Figure 2.

Figure 2

Effect of suboptimal fixation conditions on actin and CD4 organization. (A) NanoJ-Fluidics protocol to perform the live-to-fixed cell correlation under different suboptimal fixation conditions. (B) Epifluorescence and SIM imaging of COS7 cell expressing Utr(CH)-GFP and CD4-TagRFP-T live (Live) and fixed (Fixed) with 4% PFA in PBS at 23°C and corresponding NanoJ-SQUIRREL error maps (Error map). (C) same as in (B) but fixation was performed with 4% PFA in PEM at 4°C. (D) same as in (B) but fixation was performed with 4% PFA in PEM at 37°C. Scale bars are 10 μm.