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. 2019 Apr 5;10:675. doi: 10.3389/fimmu.2019.00675

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Copyright © 2019 Pereira, Albrecht, Culley, Jacobs, Marsh, Mercer and Henriques.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Correlation between CD4 membrane organization and actin structure fidelity upon fixation (A) NanoJ-Fluidics protocol to perform live-to-fixed cell correlation under different fixation conditions. (B) CD4 STORM imaging after fixation in different conditions (Top) and FRC map of the same region (Bottom). Scale bars are 5 μm. (C) CD4 cluster size and cluster density under different fixation conditions. (D) Diffraction limited (TIRF) live-to-fixed cell correlation using different fixation conditions. Red arrowheads highlight areas where actin disappeared upon fixation. Yellow arrowheads highlight areas where there is a difference in actin organization due to fixation. Scale bars are 1 μm. ^****p < 0.001.