FIGURE 2.

Effect of Si nutrition on ROS accumulation in leaves of iron-deficient barley seedlings. Representative fluorescent microscopy images of the youngest fully expanded leaves showing ROS accumulation as green fluorescence with the probe H₂DCF DA. The plants were treated as described in the legend of Figure 1 (C- control plants grown optimally supplied with Fe; -Si – plants grown in the absence of Fe and Si; +Si – plants grown in the absence of Fe and supplied with Si). Scale bar = 40 μm.