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. 2019 Apr 9;13:1135–1144. doi: 10.2147/DDDT.S194182

Figure 2.

Figure 2

Curcumin inhibits autophagy and HIF-1α in the OGD/R model.

Notes: (A) PC12 cells were treated with curcumin under OGD/R conditions. LC3 II, LC3 I, P62, and HIF-1α expressions were detected using Western blot. (B–D) Figures showing LC3 II/LC3 I (B), P62/GAPDH (C), HIF-1α/GAPDH (D) in each group. Data were represented as the mean ± SD, n=3; *P<0.05 and **P<0.01 vs normal group; #P<0.05 and ##P<0.01 vs OGD/R control group. (E) Autophagic compartments of PC12 cells under OGD/R and curcumin treatment conditions detected using fluorescence microscopy. Scale bar =50 μm, magnification 400×. The above-mentioned column shows Hoechst 33342 Nuclear Stain (blue), the middle shows autophagic compartment stain (green), and the bottom shows merged images. (F) PC12 cells were transfected with HIF-1α siRNA under either normal or OGD/R conditions. HIF-1α expression level was detected using Western blot. Data were represented as the mean ± SD, n=3; *P<0.05 and **P<0.01 vs normal control group; ###P<0.001 vs OGD/R control group.

Abbreviations: Con, control; Cur, curcumin; OGD/R, oxygen glucose deprivation/reperfusion; ncRNA, negative control siRNA; HIF-1α siRNA, hypoxia inducible factor-1α siRNA; ns, not significant; LC3 II, light chain 3-II; LC3 I, light chain 3-I.