Figure 3.

Role of cortactin in Src-mediated modulation of AK23-induced loss of cell cohesion. (A) Cortactin is important for cell cohesion, indicated by enhanced fragmentation of cortactin-deficient (CTTN^−/−) cell monolayers in dispase-based dissociation assay (n = 5; *p < 0.05 vs. control). (B) Confirmation of cortactin knockout by Western blot, α-Tubulin served as loading control (n = 3). (C,D) CTTN^−/− and wt mouse keratinocytes exposed to a dissociation assay following either short time (2 h) or long time (24 h) incubation with AK23 (n > 7, ^#p < 0.05; *p < 0.05 vs. control). (E) Reconstitution of cell adhesion measured by dissociation assay after Ca²⁺ switch in CTTN^−/− and wt keratinocytes in absence or presence of Src inhibitor PP2 (n > 7, ^#p < 0.05; *p < 0.05 vs. control).