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. 2019 Apr 8;8(1):1596668. doi: 10.1080/20013078.2019.1596668

Figure 2.

Figure 2.

Morphology and size distribution of ALIX/CD63 positive sEVs and sEVCs in CRC tissue. (a) Violin plot shows the diameter distribution of extracellular sEVCs (left) and distinguishable ALIX-positive spheroids inside and close to the sEVCs (right) (measured with Pannoramic Viewer 1.15.3 and Leica LAS X 3.1.1 software). (b) Comparison of the diameters of the at least partially extracellularly localized ALIX/CD63 positive structures in migrating CRC cells. (c) Comparison of the diameters of similarly localized CD63-positive structures in CRC tissues and in HT29 culture. (d) Average number of the at least partially extracellular ALIX-positive or CD63-positive signals in migrating CRC cells (tissue). (e) Average number of intracellularly localized ALIX-positive or CD63-positive signals in migrating CRC cells (tissue). B-E shows mean±S.D. of n = 40 cells. HyVolution (f) and STED (g) microscopic images of an ALIX positive (red) sEVC (white box) which is partially embedded within the CK positive cytoplasm (green) of the migrating CRC cell. Scale bars: 5 μm and 2 μm respectively. (h) Fluorescence intensity profile of ALIX positive sEVC (red) crossed by a selected line (green). Scale bar: 1.5 μm. (i) Fluorescence intensity profile and measured Full-Width at Half-Maximum (FWHM) values of a small group ALIX positive sEVs and (j) a single ALIX positive sEV. Scale bars: 0.2 µm and 0.15 μm (200 nm and 150 nm) respectively. FWHM values: 141 and 149 nm, respectively.