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. 2019 Apr 11;8(1):1597614. doi: 10.1080/20013078.2019.1597614

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterization of exosomes isolated from N-Myc amplified and non-amplified neuroblastoma cells.

(a) Western blot analysis of exosomal enriched proteins Alix and TSG101 in fractions obtained from OptiPrep density gradient centrifugation (SK-N-BE2 cells). TSG101 and Alix were enriched in fractions 7 and 8 corresponding to the buoyant density of 1.13 and 1.15 g/mL. (b) Western blot analysis representing the presence of Alix and TSG101 that are enriched in exosomes derived from SH-SY5Y cells. Fraction 7 contained a high abundance of Alix and TSG101. (c) TEM images of exosomes isolated by OptiPrep density gradient centrifugation suggested the presence of vesicles. (D) Venn diagram representing proteins present in exosomes derived from N-Myc amplified (SK-N-BE2) and non-amplified (SH-SY5Y) neuroblastoma cells. A total of 749 proteins are found to be common between the exosomes isolated from the two neuroblastoma cell lines. (e) Venn diagram depicting differentially abundant (>2-fold) proteins in SK-N-BE2 and SH-SY5Y cell-derived exosomes. A total of 581 proteins were enriched in exosomes derived from SK-N-BE2 cells compared to the exosomes from SH-SY5Y cells. Similarly, a total of 385 proteins were enriched in exosomes isolated from SH-SY5Y cells. The red arrow represents proteins that are of high abundance in SK-N-BE2 cell-derived exosomes compared SH-SY5Y cell-derived exosomes. Green arrow represents proteins that are of lower abundance in SK-N-BE2 cell-derived exosomes compared SH-SY5Y cell-derived exosomes.