Figure 4.

Cytotoxicity evaluation of diclofenac-loaded ginsenoside Rb1 (Rb1-Dic) micelles (n = 3). An MTT assay was performed on Human ATCC CRL-11135 corneal epithelial cells (HCECs). HCECs were incubated with Rb1-Dic micelles for (A) 24 h, (B) 48 h, and (C) 72 h; (D) HCECs incubated for 1 h with Rb1-Dic micelle formulations, commercial diclofenac (Dic) eye drops, and benzalkonium chloride (BAC) as a positive control. MTT transformed crystals were dissolved in DMSO and absorbance at 490 nm was measured. Absorbances were normalized to the untreated control cultures, which represented 100% viability. (*p < .05 when compared to the Rb1-Dic micelle group).