Fig. 2. Membrane anchoring-responsive fluorescence enhancement of Chol–PEG–Cy5 for wash-free plasma membrane imaging. (a) Confocal fluorescence images of A549 cells stained using 2 μM Chol–PEG–X (X = Cy5, FITC, Rhod and NBD) without PBS washing. Scale bar = 25 μm. (b) Corresponding fluorescence intensity profiles of the marked white lines in (a). (c) Fluorescence emission spectra of Chol–PEG–Cy5 (2 μM) in different concentrations of liposome solutions. (d) Fluorescence emission spectra of Chol–PEG–Cy5 (2 μM) in 1% SDS and PBS solutions, respectively.
