FIG. 5.

Vector copy numbers and total number of ISs per mouse in SINfes.gp91s- and SF91eGFP-transplanted animals. Vector copy numbers in DNA samples obtained from PB, BM, and SP of transplanted primary (A) and secondary (B) animals. (C) Total number of ISs per mouse. ISs were obtained from serially transplanted SF91eGFP and SINfes.gp91s mice by ligation-mediated/linear-amplification-mediated PCR and subsequent 454 sequencing. (D) Percentage of ISs found in or close to the Mds1/Evi1 locus for primary and secondary transplanted mice. With SINfes.gp91s, significantly less ISs were detected in primary and in secondary animals, respectively. *p=0.077 and 0.026 for primary and secondary mice, respectively, as determined by χ²-test. BM, bone marrow; ISs, integration sites; PB, peripheral blood; SP, spleen.