Table 3. Methylation levels of CpG sites located at genes of the longevity-regulating pathways and differences between both age groups.

	CpG	Gene	<45 years	≥ 45 years	p-value
1	cg08862778	MTOR	0.111 ± 0.001	0.106 ± 0.001	0.0422
2	cg11322849	INS	0.576 ± 0.003	0.565 ± 0.003	0.0115
3	cg07199894	ULK1	0.227 ± 0.004	0.243 ± 0.003	0.0068
4	cg11658986	ADCY6	0.721 ± 0.002	0.709 ± 0,002	0.0016
5	cg14862787	CREB5	0.833 ± 0.001	0.822 ± 0.001	< 0.0001
6	cg05792022	FOXO1	0.213 ± 0.002	0.201 ± 0.002	0.0002
7	cg04149773	ADCY6	0.741 ± 0.002	0.732 ± 0.002	0.0019
8	cg14113970	CAMK4	0.774 ± 0.003	0.759 ± 0.004	0.0198
9	cg14844401	ADCY5	0.649 ± 0.002	0.641 ± 0.002	0.0343
10	cg14267811	TSC1	0.190 ± 0.003	0.177 ± 0.003	0.0049
11	cg01284192	IGF1R	0.801 ± 0.002	0.792 ± 0.002	0.0051
12	cg24061580	PRKAG2	0.217 ± 0.002	0.209 ± 0.001	0.0067
13	cg06223834	ADCY9	0.773 ± 0.003	0.758 ± 0.003	0.0009
14	cg19503731	AKT3	0.865 ± 0.001	0.860 ± 0.001	0.0401
15	cg13154908	PIK3CA	0.798 ± 0.003	0.788 ± 0.003	0.0497
16	cg18237616	RHEB	0.840 ± 0.002	0.830 ± 0.002	0.0039
17	cg14323456	RHEB	0.821 ± 0.003	0.810 ± 0.004	0.0456
18	cg01781374	CAMK4	0.831 ± 0.002	0.822 ± 0.002	0.0099
19	cg11301281	CREB5	0.763 ± 0.001	0.752 ± 0.001	< 0.0001
20	cg02823066	IGF1	0.658 ± 0.002	0.647 ± 0.002	0.0028
21	cg20300093	ADCY5	0.618 ± 0.002	0.609 ± 0.002	0.0032
22	cg14077232	EHMT1	0.823 ± 0.002	0.812 ± 0.002	0.0026
23	cg08128650	RELA	0.777 ± 0.001	0.766 ± 0.002	0.0001
24	cg19418273	CREB3L2	0.844 ± 0.002	0.835 ± 0.003	0.0316
25	cg14072989	RPS6KB1	0.825 ± 0.002	0.814 ± 0.003	0.0308

CpG beta values are grouped by age and p values are shown. Data are expressed as beta values mean ± SEM.