Table 4. Methylation levels of 25 CpG sites located at genes of the longevity-regulating pathways that were differentially methylated between younger (<45 y) and older subjects (≥45 y) in GEO datasets. GSE76399 compared insulin resistant and insulin sensitive individuals in PBMC, whereas GSE65057 compared obese and non-obese individuals in liver samples.
| PMBC (GSE76399) | Liver tissue (GSE65057) | ||||||
| CpG | Gene | Ins- Sens (n=40) | Ins- Resist (n=40) | P value | Non-Obese (n=7) | Obese (n=8) | P value |
| cg08862778 | MTOR | 0.022 ± 0.001 | 0.024 ± 0.001 | 0.145 | 0.069 ± 0.004 | 0.079 ± 0.009 | 0.357 |
| cg11322849 | INS | 0.692 ± 0.006 | 0.668 ± 0.016 | 0.154 | 0.676 ± 0.008 | 0.626 ± 0.017 | 0.025 |
| cg07199894 | ULK1 | 0.199 ± 0.006 | 0.206 ± 0.007 | 0.447 | 0.319 ± 0.011 | 0.311 ± 0.014 | 0.661 |
| cg11658986 | ADCY6 | 0.675 ± 0.008 | 0.677 ± 0.007 | 0.893 | 0.621 ± 0.010 | 0.566 ± 0.010 | 0.003 |
| cg14862787 | CREB5 | 0.875 ± 0.003 | 0.856 ± 0.016 | 0.255 | 0.719 ± 0.011 | 0.720 ± 0.020 | 0.968 |
| cg05792022 | FOXO1 | 0.209 ± 0.004 | 0.204 ± 0.005 | 0.470 | 0.261 ± 0.007 | 0.237 ± 0.014 | 0.170 |
| cg04149773 | ADCY6 | 0.724 ± 0.018 | 0.726 ± 0.019 | 0.959 | 0.797 ± 0.006 | 0.748 ± 0.019 | 0.035 |
| cg14113970 | CAMK4 | 0.881 ± 0.027 | 0.827 ± 0.037 | 0.236 | 0.850 ± 0.008 | 0.795 ± 0.026 | 0.078 |
| cg14844401 | ADCY5 | 0.698 ± 0.010 | 0.658 ± 0.028 | 0.186 | 0.657 ± 0.011 | 0.631 ± 0.016 | 0.202 |
| cg14267811 | TSC1 | 0.209 ± 0.005 | 0.211 ± 0.005 | 0.775 | 0.219 ± 0.006 | 0.212 ± 0.012 | 0.605 |
| cg01284192 | IGF1R | 0.842 ± 0.005 | 0.837 ± 0.005 | 0.471 | 0.815 ± 0.009 | 0.774 ± 0.009 | 0.008 |
| cg24061580 | PRKAG2 | 0.199 ± 0.004 | 0.186 ± 0.005 | 0.060 | 0.247 ± 0.004 | 0.244 ± 0.008 | 0.760 |
| cg06223834 | ADCY9 | 0.867 ± 0.006 | 0.821 ± 0.030 | 0.143 | 0.838 ± 0.010 | 0.795 ± 0.015 | 0.042 |
| cg19503731 | AKT3 | 0.953 ± 0.003 | 0.946 ± 0.003 | 0.086 | 0.902 ± 0.006 | 0.895 ± 0.010 | 0.578 |
| cg13154908 | PIK3CA | 0.955 ± 0.003 | 0.949 ± 0.003 | 0.087 | 0.927 ± 0.004 | 0.909 ± 0.006 | 0.029 |
| cg18237616 | RHEB | 0.919 ± 0.018 | 0.914 ± 0.018 | 0.860 | 0.712 ± 0.017 | 0.717 ± 0.015 | 0.819 |
| cg14323456 | RHEB | 0.956 ± 0.004 | 0.956 ± 0.003 | 0.958 | 0.929 ± 0.010 | 0.886 ± 0.015 | 0.039 |
| cg01781374 | CAMK4 | 0.926 ± 0.016 | 0.878 ± 0.023 | 0.097 | 0.839 ± 0.012 | 0.852 ± 0.012 | 0.457 |
| cg11301281 | CREB5 | 0.768 ± 0.019 | 0.733 ± 0.024 | 0.256 | 0.636 ± 0.011 | 0.584 ± 0.016 | 0.024 |
| cg02823066 | IGF1 | 0.649 ± 0.021 | 0.619 ± 0.037 | 0.481 | 0.319 ± 0.015 | 0.323 ± 0.013 | 0.830 |
| cg20300093 | ADCY5 | 0.639 ± 0.017 | 0.594 ± 0.028 | 0.172 | 0.708 ± 0.009 | 0.647 ± 0.027 | 0.068 |
| cg14077232 | EHMT1 | 0.954 ± 0.002 | 0.956 ± 0.002 | 0.479 | 0.905 ± 0.005 | 0.901 ± 0.013 | 0.806 |
| cg08128650 | RELA | 0.799 ± 0.011 | 0.768 ± 0.017 | 0.138 | 0.698 ± 0.006 | 0.666 ± 0.015 | 0.077 |
| cg19418273 | CREB3L2 | 0.964 ± 0.003 | 0.960 ± 0.004 | 0.527 | 0.940 ± 0.005 | 0.907 ± 0.007 | 0.003 |
| cg14072989 | RPS6KB1 | 0.948 ± 0.006 | 0.947 ± 0.006 | 0.994 | 0.904 ± 0.013 | 0.839 ± 0.023 | 0.033 |
Ins-Sens: Insulin sensitive; Ins-Resist: Insulin Resistant. PBMC: Peripheral Blood Mononuclear Cells. Blue boxes represent significant changes in DNAm (p<0.05).