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. 2019 Mar 26;11(6):1759–1777. doi: 10.18632/aging.101867

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Copyright © 2019 Choi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mechanistic target of maturation promoting factor (MPF) signaling is dysregulated in TubA-treated oocytes. (A) Cyclin-dependent kinase (Cdk1, 2, 4, 6, Cdc25b, and Ccnb1) mRNA expression profiles using IGV. (B) Confocal immunofluorescence analysis of CDK1 (green) and Cyclin B (red) in oocytes with/without TubA exposure during in vitro maturation at 0 h, 9 h, and 12 h. The experiment was repeated three times. DNA was stained with DAPI (blue). Right indicates quantification of each staining density. (C, D) mRNA expression profiles of cytokinesis- and MI arrest-related factors in mouse oocyte profiles in matched pairs of TubA (10 or 20 μM)-treated (TubA/GV pairs) versus untreated control groups (MII/GV pairs). (E) Confirmation of DEGs obtained from RNA-seq. Bar graph showing relative gene expression obtained from qRT-PCR analysis of selected genes. *, **, ***, and **** represent genes displaying statistically significant changes in expressions.