Extended Data 8. Genetic inhibition of IL23 limits castration resistance in Pten^pc-/- mice.

a, Experimental set-up. Sham-operated (Sham) or Castrated (CTX) Pten^pc-/- mice were lethally irradiated and transplanted with BM precursors depleted by T, B, and NK cells from IL23^wt and IL23^ko mice. Then, monitored by MRI for tumor progression. b, Representative dot plot of BM precursors pre- and post-depletion of T, B, and NK cells. Data were validated in two biological independent experiments. c, Quantification of the tumor size of Sham-operated Pten^pc-/-;IL23^wt (n=4) and Pten^pc-/-;IL23^ko (n=4), and castrated Pten^pc-/-;IL23^wt (n=4) and Pten^pc-/-;IL23^ko (n=7) mice at completion of the study is reported as fold increase of the prostate anterior lobe (AL) volume (fold change compared with CTX Pten^pc-/-;IL23^wt group). Data are reported as mean ± SE. Statistical analyses (Unpaired two sided t test): **P <0.01, ***P <0.001. d, H&E, Ki-67, and pSTAT3^Y705 immunohistochemical staining (Ki-67 and pSTAT3^Y705 brown; nuclei, blue) of one representative Pten^pc-/-;IL23^wt and Pten^pc-/-;IL23^ko mouse of at least the three analyzed at completion of the study. Scale Bar 50 or 25 μM.