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. 2019 Apr 12;14(4):e0215205. doi: 10.1371/journal.pone.0215205

Fig 9. Detection of ER stress response.

Fig 9

(A) Expression of related proteins, including eIF2α, p-eIF2α, CHOP, IRE1α, p-IRE1α, and Bcl-2, were measured by Western blotting using HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, the relative levels of the six proteins were calculated based on the intensity of actin protein. Five or six mice per group were assayed in triplicate by Western blotting. (B) Changes in the mRNA level of the XBP-1 and GADD34 in the NT and Tg mice. The transcription level of the XBP-1 and GADD34 gene were examined by RT‐PCR using specific primers. Data are reported as the mean ± SD. *, p<0.05 compared to the NT mice. (C) Ultrastructure of the ER in Paneth cells was detected by TEM at 4,000× magnification. N, nucleus; ER, endoplasmic reticulum.