Fig 1. Expression of the RHA1B gene in G. pallida and localization of the RHA1B protein in plant cells.

(A) Diagram of the RHA1B protein indicating the signal peptide and RING-H2 domains. (B) RHA1B is expressed in the dorsal esophageal gland. In situ hybridization of sections of G. pallida second-stage juveniles (J2) with the antisense probe binding to the dorsal gland cells of para-J2s. No signal was observed in pre-J2s incubated with the sense and anti-sense probes. dg–dorsal gland, s–stylet. (C) Subcellular localization of free GFP and GFP- ^ΔSPRHA1B fusion protein in N. benthamiana leaves 36 hours after agro-infiltration. (B, C) Experiments were repeated three times with similar results.