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. 2019 Apr 12;15(4):e1007720. doi: 10.1371/journal.ppat.1007720

Fig 4. RHA1B suppresses PTI and ETI signaling in plants.

Fig 4

(A) RHA1B suppresses expression of two PTI marker genes NbAcre31 and NbWRKY22 induced by flg22 in an E3-independent manner. The relative gene expression of NbAcre31 and NbWRKY22 was measured by qRT-PCR at one hour after flg22 treatment. The NbEF1 gene was used as internal reference gene. Error bars are s.e.m., n = 3 biological replicates. (B) RHA1B suppresses HR mediated by a broad range of NB-LRR immune receptors. N. benthamiana leaves were agro-infiltrated with combination of either ΔSPRHA1B or empty vector with auto-active NB-LRR mutant (PrfD1416V, Rx1D460V or Rpi-blb1D475V), NB-LRR receptor and its cognate avirulence effector (Gpa2/RBP1 or Bs4/AvrBs4), or the pro-apoptotic mouse protein Bax and MAPKKKα at appropriate inoculum. Photographs were taken 5 days after agro-injection. (A, B) Experiments was repeated at least three times with similar results.