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. 2019 Apr 12;14(4):e0215391. doi: 10.1371/journal.pone.0215391

Fig 5. Schematic illustration of the designed lentiviral vectors used to transduce the cells with the genetic constructions for the expression of LaFr26, oxyopinin-2b, and mCherry.

Fig 5

Lv-LaFr26 (A) and Lv-Oxy-2b (B) carry IRES for the bicistronic coexpression of GFP and the spider peptides, which were designed to be secreted byLaFr26 or oxyopinin-2b. (C) Structure of Lv-mCherry. The expression cassettes were inserted in the downstream of RNA packaging signal between two long terminal repeats. (β-actin, promoter of chick β-actin used for the coexpression; GFP, green fluorescent protein gene; IRES, internal ribosomal entry site for the bicistronic coexpression; Signal, Gaussia luciferase signal peptide; mCherry, mCherry gene) (D) Schematic illustration of the autotoxic expression of the venom peptides with Lv-LaFr26 and Lv-Oxy-2b.