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. Author manuscript; available in PMC: 2019 Apr 12.
Published in final edited form as: Cell Stem Cell. 2016 Dec 29;20(3):385–396.e3. doi: 10.1016/j.stem.2016.11.017

Figure 3. PTEN deletion induces expansion and folding of forebrain neuroepithelia in human cerebral organoids.

Figure 3.

A-C) Immuno-staining for NPs using antibodies against Nestin (A), Pax6 (B), and Sox2 (C) in control and mutant WIBR3 organoids at 4 weeks. Scale bars, 50um (A-B) and 20um (C).

D-G) Light sheet images and quantification of control and mutant WIBR3 organoids treated with dorsomorphin for 14 days, demonstrating expansion and folding (D), increased folds density (G), patterning towards the forebrain fate (E) at the preclusion of none forebrain lineages (F).

Results are mean +/− SEM. **p<0.01, ***p<0.001. See also Figure S4.