KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| See Table S1 | This study | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dorsomorphin | Stemgent | 04–0024 |
| GDC-0068 | Selleckchem | S2808 |
| MK-2206 | Selleckchem | S1078 |
| Critical Commercial Assays | ||
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | G7571 |
| CellEvent Caspase-3/7 Green Detection Reagent | Invitrogen | C10423 |
| Click-iT EdU Imaging Kit | Invitrogen | C10337 |
| TSA Plus Cyanine 3 System | Perkin Elmer | NEL744001KT |
| Experimental Models: Cell Lines | ||
| Human: WIBR1/2/3 hESCs | (Lengner et al., 2010) | N/A |
| Human: WIBR1/2/3 PTEN mutant hESCs | This study | N/A |
| Mouse: V6.5 mESCs | (Stelzer et al., 2015) | N/A |
| Mouse: V6.5 Pten mutant mESCs | This study | N/A |
| Sequence-Based Reagents | ||
| Primers: see Table S2 | This study | N/A |