Figure 1. Evolution of the TRPM2 proteins in the Animalia kingdom.
Evolutional progression through major animal taxa is indicated to the left, orange arrow marks the appearance of vertebrae. Chosen TRPM2 sequences from the indicated species are aligned in their Pore helix/Filter/Post-filter helix region (left, respective structural segments identified by bars on top and bottom) and Nudix box region (right); respective residue numbering is shown. The taxa Porifera (sponges; marked with '+') and Ctenophora (comb jellies; marked with '#') did not return any TRPM2-like sequences in BLAST. Asterisks mark Nudix box residues critical for ADPR hydrolysis. Concerted changes that happened between chordates and vertebrates in the sequences of the filter, post-filter helix, and the Nudix box are highlighted in blue (invertebrates) and red (vertebrates). Several of the listed proteins are predicted or uncharacterized proteins. For additional details on the chosen sequences, see Table in Figure 1—figure supplement 1.
Figure 1—figure supplement 1. Evolutional progression of the TRPM2 proteins through the Animalia kingdom and the associated changes in their Pore/Filter and Nudix box regions.
