Figure 4.

C1 and C4 Prevent Adenoviral Protein VI Exposure and Endosomal Escape

(A) IP of Ad5 with 9C12 after Ad5 was incubated at the indicated temperatures for 30 min. WB: anti-adenovirus.

(B) IP after Ad5 and 9C12 were complexed with C1 or C1/C4 and then incubated at 37°C or 49°C for 30 min. WB: anti-adenovirus.

(C and D) HeLa TRIM21 KO cells were infected for 30 min in the presence of 9C12 or 9C12+ complement. Error bars depict the mean ± SEM of the indicated number of cells (n) acquired in three independent experiments. Scale bar, 5 μm.

(C) Left: Ad5 staining is displayed in green; protein VI staining is depicted in red. Right: quantification of protein VI puncta per cell in the indicated conditions.

(D) Left: Ad5 staining is displayed in green; Galectin-3 staining is depicted in red. Right: quantification of Galectin-3 puncta per cell in the indicated conditions.

Original western blots are included in Figure S6.