REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti adenovirus protein VI (affinity purified)	Urs F. Greber (Burckhardt et al., 2011)	N/A
Goat anti-Rabbit IgG (H+L) AF647	Thermo Fisher Scientific	Cat# A-21245; RRID: AB_2535813; Dil: 1:500
Galectin 3 Monoclonal Antibody (M3/38) PE	Thermo Fisher Scientific/ eBioscience	Cat# BMS1043; RRID: AB_10597892; 1μg/mL
Anti-Human IgG (Fab specific)-FITC antibody produced in goat	Sigma-Aldrich	Cat# F5512; RRID: AB_259649; Dil: 1:200
Anti-Human IgG (Fab specific)- antibody produced in goat	Sigma-Aldrich	Cat# I5260; RRID: AB_260206; Dil: 1:200
Donkey Anti-Goat IgG (H+L) Antibody, AF647	Thermo Fisher Scientific	Cat# A-21447; RRID: AB_141844; Dil: 1:1000
Lamin B1 antibody	Abcam	Cat# ab16048; RRID: AB_10107828; Dil: 1:1000
Goat Anti-Rabbit IgG (H+L) Antibody AF568	Thermo Fisher Scientific	Cat# A-11011; RRID: AB_143157; Dil: 1:1000
Anti-Adenovirus antibody	Millipore	Cat# AB1056; RRID: AB_90213; Dil: 1:1000
52 kDa Ro/SSA (D-12) antibody (TRIM21)	Santa Cruz Biotechnology	Cat# sc-25351; RRID: AB_628286
Anti-C4b antibody	Abcam	ab181241; Dil: 1:1000
β-Actin Antibody (C4)	Santa Cruz Biotechnology	Cat# sc-47778 HRP; RRID: AB_2714189; 0.04μg/mL
Rabbit anti goat IgG (H/L) antibody	Bio-Rad / AbD Serotec	Cat# 5160-2104; RRID: AB_619999; Dil: 1:5000
Anti-Mouse IgG (whole molecule)-Peroxidase antibody produced in rabbit	Sigma-Aldrich	Cat# A9044; RRID: AB_258431; Dil: 1:5000
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat	Sigma-Aldrich	Cat# A0545; RRID: AB_257896; Dil: 1:5000
6X His tag antibody [HIS-1] (Alkaline Phosphatase)	Abcam	Cat# ab49746; RRID: AB_867457; Dil:1:5000
C1q complement antibody	Dako	Cat# A0136; RRID: AB_2335698; Dil:1:10000
Rabbit IgG HRP Linked Whole Ab antibody	GE Healthcare	Cat# GENA934; RRID: AB_2722659; Dil:1:10000
Anti human IgG (Fc-specifc) AP linked antibody	Sigma-Aldrich	Cat#:A9544; RRID: AB_258459; Dil: 1:5000
iTAg Tetramer/PE - H-2 Kb OVA (SIINFEKL)	MBL	Cat# TB-5001-1; 2μL/sample
Bacterial and Virus Strains
Adenovirus 5 (E1/E3 deleted)	ViraQuest	VQAd CMV eGFP/mCherry
Chemicals, Peptides, and Recombinant Proteins
Complement serum	CompTech	Cat#NHS
C1q depleted serum	CompTech	Cat#A300
C2 depleted serum	CompTech	Cat#A312
C3 depleted serum	CompTech	Cat#A314
C4 depleted serum	CompTech	Cat#A308
C1q	CompTech	Cat#A099
C1	CompTech	Cat#A098
C4	CompTech	Cat#A105
Adenovirus Type 5 Hexon	BioRad	MPP002
Critical Commercial Assays
Human Complement C4 ELISA Kit	abcam	Cat#ab108825
RNeasy Micro Kit	Qiagen	Cat#74004
DNeasy Blood & Tissue Kit	Qiagen	Cat# 69504
Deposited Data
Raw and analyzed data	(Bottermann et al., 2018)	GEO: GSE119119
Experimental Models: Cell Lines
HEK293T	ATCC	Cat# CRL-3216; RRID: CVCL_0063
HeLa	ATCC	Cat# CCL-2; RRID: CVCL_0030
THP-1	ATCC	Cat#:TIB-202; RRID: CVCL_0006
Experimental Models: Organisms/Strains
C57BL/6J	The Jackson Laboratory	JAX:000664; RRID: IMSR_JAX:000664
C57BL/6-Trim21tm1Hm/J	(Yoshimi et al., 2010)	JAX:010724; RRID: IMSR_JAX:010724
B6.129S4-C4tm1Crr/J	Provided by Michael C. Carroll	JAX:003643; RRID: IMSR_JAX:003643
Oligonucleotides
TRIM21 targeting gRNA; 5’ATGCTCACAGGCT CCACGAA3’	(McEwan et al., 2017)	N/A
Ad5 Taqman probe; Ad5 for: TTGCGTCGGTG TTGGAGA, Ad5 rev: AGGCCAAGATCGTGAA GAACC, Ad5 probe: FAM-CTGCACCACAT TTCGGCCCCAC-TAMRA	(Bottermann et al., 2018)	N/A
CAR siRNA	Santa Cruz	sc-29906
Control siRNA	Santa Cruz	sc-37007
Recombinant DNA
pSpCas9(BB)-2A-GFP (PX458)	(Ran et al., 2013)	Addgene #48138
Software and Algorithms
FlowJo, LLC	Tree Star	https://www.flowjo.com/solutions/flowjo/downloads
GraphPad Prism version 7.00	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Fiji	(Schindelin et al., 2012)	https://fiji.sc/#download
ImageJ	(Schneider et al., 2012)	https://imagej.nih.gov/ij/download.html
Image J plugin ComDet v0.3.7	Eugene Katrukha	https://imagej.net/Spots_colocalization_(ComDet)