Table 1.
IC50 values (in nM) for AgrC antagonism by selected phase-one n7OFF analogs in S. aureus groups-I−IVa. 95% confidence intervals (CI) in parentheses.
| compound | group-I | group-II | group-III | group-IV |
|---|---|---|---|---|
| n7OFF | 181 (154−213) | 583 (468−725) | 332 (280−395) | 5938 (4221−8532) |
| n7OF(3fF) | 30.6 (22.5–41.8) | 445 (217–911) | 222 (158–312) | ~3500 (ND)b |
| n7OF(3ClF) | 125 (90–173) | 167 (118–237) | 630 (364–1090) | 2240 (1150–4460) |
| n7OChaF | 439 (278–695) | 627 (317–1238) | 194 (148–255) | 1250 (990–1580) |
| n7OCpaF | 141 (107–185) | 204 (169–246) | 19.7 (14.8–26.3) | 498 (414–598) |
| Bn-n7OFF | 15.3 (12.4–19.0) | 37.8 (29.8–47.9) | 17.3 (14.1–21.2) | 564 (435–731) |
| Et-n7OFF | 129 (97.7–170) | 197 (152–255) | 531 (312–905) | --c |
| iPr-n7OFF | 45.8 (34.0–61.9) | 97.1 (65.6–144) | 316 (192–522) | --c |
| Pr-n7OFF | 57.9 (30.3–111) | 103 (68–155) | 203 (121–341) | --c |
| iBu-n7OFF | 56.0 (38.6–81.2) | 40.7 (35.3–46.9) | 55.3 (37.7–81.0) | --c |
| Bu-n7OFF | 34.7 (28.1–42.8) | 72.3 (45.6–115) | 78.1 (43.6–140) | --c |
| pentyl-n7OFF | 15.8 (13.5–18.4) | 17.0 (14.7–19.6) | 15.7 (11.8–21.0) | --c |
| Ph-n7OFF | 157 (123–201) | 23.0 (15.6–34.1) | 98.2 (75.0–129) | --c |
| n7OF(4fF) | 375 (204–689) | 354 (253–497) | 2140 (1080–4220) | --c |
a
Data for parent n7OFF included for comparison. See Methods section for details of strains and assays. Comprehensive assay data for all compounds tested are provided in Tables S2−S3.
b
The confidence interval was too wide to quantify accurately.
c
Minimal activity at concentrations up to 10 μM; IC50 not calculated.