Figure 3.

MitoTracker® staining is brighter in CD4⁺ T cells than CD8⁺ T cells. (A) MT deep red and MT green staining was compared between CD4⁺ and CD8⁺ T cells from the same individual (n = 8) using both MFI and the objective gating method (cells in the top 90% of the fluorescence range, or MT₉₀). (B) By both methods, MitoTracker® fluorescence was consistently higher in CD4⁺ T cells than CD8⁺ T cells. Data analyzed using Wilcoxon matched‐pairs signed rank test. AU, arbitrary units; MFI, median fluorescence intensity; MT, MitoTracker®.