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. 2018 Dec 21;95(4):450–456. doi: 10.1002/cyto.a.23705

Table 1.

Both MT90 and MT50 gating show significant correlation with MFI when comparing CD4+ and CD8+ T cells in the same participant

MitoTracker® Deep Red MitoTracker® Green
MTDR50 vs. MFI MTDR90 vs. MFI MTDR99 vs. MFI MTG50 vs. MFI MTG90 vs. MFI MTG99 vs. MFI
Spearman r 0.79 0.83 0.69 0.83 0.88 0.59
P value 0.028 0.015 0.069 0.015 0.007 0.134

To examine the correlation between MT gating and MFI, the within‐participant fold‐change in MitoTracker® MFI in CD4+ T cells compared with CD8+ T cells (CD4 MFI/CD8 MFI) was calculated and then correlated with the fold‐change (CD4 MT/CD8 MT) in MT50, MT90, and MT99 for each sample shown in Figure 3. Correlations performed using Spearman's rank correlation. Bold text indicates significant correlations (two‐tailed P value <0.05).