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Heavy heparin disaccharides prepared by enzymatic depolymerization of non-anticoagulant perdeuteroheparin and precursors. a) Chemoenzymatic synthesis and depolymerization scheme for perdeuteroheparin disaccharide standards. Unsaturated uronic acid residues in disaccharide products of heparin lyases I, II, III are indicated with slash at non-reducing end. b) Retention time and m/z of 2-aminoacridone-labeled USP and heavy heparin dp2 standards (Supplementary Fig. 4).
