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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2019 Mar 29;58(18):5910–5914. doi: 10.1002/anie.201812892

Figure 3.

Figure 3.

High-quality single cell mass spectra and ICC images are acquired and compared for over a thousand individual cells. Single cell spectra for cells displayed in each panel are shown. (a) Single cell spectrum for an NF-L-positive cell. (b) A second NF-L positive cell with similar mass spectral features. (c) GFAP-positive single cell that is visibly different from the (d) second chosen GFAP-positive cell. Green and red fluorescence corresponds to NF-L and GFAP, respectively, while blue fluorecsence corresponds to a nuclear dye.(e) t-SNE plot of NF-L-positive cells are colored to correspond to the five clusters determined by HCA. (f) t-SNE plot of GFAP-positive cells colored to correspond to the four clusters determined by HCA.