Figure 8:

Example of bioinformatics-driven capsid design. The reconstructed ancestral phylogeny of AAV was used to develop a new mutant, Anc80. Anc80 is the ancestor of currently studied AAV serotypes excluding AAV4 and AAV5. The distance between viruses in the hierarchical chart denotes relative distance of relation between the viruses. Figure reprinted with permission from “In Silico Reconstruction of the Viral Evolutionary Lineage Yields a Potent Gene Therapy Vector,” by E. Zinn et al, 2015, Cell Reports. Copyright 2015 by Elsevier.