Figure 1.

Amplification, hybridization, and washing take place on a Lab-on-a-Film disposable, which includes 203 hemispherical gel elements on a thin film substrate. Initially, master mix and sample are introduced into the reaction chamber. Subsequently, asymmetric PCR occurs in the chamber, resulting in a mix of fluorescently-labeled, single-stranded amplicons. Hybridization of the labeled amplicons to the gel elements follows, but this does not require a fluidic transfer step. The unbound molecules are then washed away using a pipettor, and the liquid is imbibed into an absorbent in the waste chamber. The last step is fluorescence imaging and analysis.