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. 2019 Mar 27;14:1–14. doi: 10.1016/j.omto.2019.03.005

Figure 2.

Figure 2

Effects of siRNA-Mediated Knockdown of Cellular RRM1 and TK1 on Replication of VACVs

(A) Western blot detection of RRM1 (left) and TK1 (right) protein expression in LoVo cells 24, 48, and 72 h after transfection with specific siRNA and non-targeting control siRNA (siRNAc). Molecular weight standards are shown (M). The western blots are representative of three different experiments. (B) Virus production in RRM1 and TK1-knockdown LoVo cells following infection with the indicated VACVs at MOI 10−3 for 48 h before performing plaque assays. The data represent the average of three independent experiments and are shown as the mean ± SD. *p < 0.05 compared to cells transfected with the non-targeting siRNAc.