Figure 2.

Co-localization of MGP and GGCX Proteins in Vesicular Structures of Epithelial Cells and Spermatozoa in the Epididymis

(A) Representative projections of stacks of serial confocal images showing the double-immunofluorescence labeling of MGP (red) and GGCX (green) in the different regions of the rat epididymis. CD, cauda epididymidis; CPS, corpus; CPT, caput; IS, initial segment. (L) lumen; (I) interstitial tissue. Scale bar, 20 μm.

(B) Three-dimensional reconstruction and orthogonal views (xy, xz, yz) of higher magnification of stacks of confocal images demonstrating the co-localization of MGP and GGCX (yellow) in both intracellular and extracellular vesicular structures of epithelial cells (arrows) and cytoplasmic droplets (double arrows) of spermatozoa in the lumen of CPS and CD. Long arrows indicate the xy, xz, and yz dimensions. DAPI (blue) was used to visualize nuclei and sperm heads. (L), lumen.

Scale bars, 10 μm.