Figure 6.

Decreased Protein Carboxylation in the Epididymis of Warfarin-Treated Rats

(A) Double immunolabeling with antibodies against Gla-residue-containing proteins (red) and MGP (green) in CPS epididymides showing the similar cellular localization of Gla residues and MGP. Enriched co-localization (yellow) of Gla-residue-containing proteins and MGP in the apical pole of principal cells and luminal vesicular contents (inset).

(B) Immunoblot of Gla-residue-containing proteins in rat epididymal and kidney lysates. Arrow indicates the expected size of MGP at ∼12 kDa. Actin: loading control.

(C) Immunolabeling of Gla-residue-containing proteins (red) in CPS epididymides of rats from warfarin-treated and control groups. Blue: DAPI labeling for nuclei. (L), lumen.

Scale bars, 20 μm in A and C, 10 μm in the inset.