Figure 6.

Cellular Uptake Mechanism of 3 and 5

(A) Energy-dependent pathways of cellular internalization of NPs.

(B–E) Cellular uptake mechanism of 3 (B and D) and 5 (C and E) in HeLa (B and C) and MCF-7 (D and E) cells. The cells were treated with 3 or 5 (5 μg/mL) for 30 min, at 37°C, 4°C, and 37°C while pre-treating with DCA (15 mM), CPZ (10 μg/mL), MβCD (10 mg/mL), and AMR (75 μg/mL) for 1 h. The cellular uptake was reflected by MFI of green fluorescence. Data were presented as mean ± SD (n = 5). Scale bar, 100 μm.