Figure 8.
Characterization of Mitochondrial Membrane Potential (ΔΨ) Reduction and Lysosomal Membrane Permeabilization (LMP) Induced by 3 and 5
(A and B) Laser scanning confocal images of cells stained with JC-1 (10 μg/mL, 10 min) after being incubated with 3 or 5 (0.2 μM, BODIPY equiv.) with green LED irradiation (40 mW/cm2) for 0 min (A) and 4 min (B), respectively. ΔΨ reduction was reflected by the MFI ratios of green and red fluorescence. Data are presented as mean ± SD (n = 5). Scale bar, 50 μm.
(C and D) Laser scanning confocal images of cells stained with AO (5 μg/mL, 10 min) after being incubated with 3 or 5 (0.2 μM, BODIPY equiv.) with green LED irradiation (40 mW/cm2) for 0 min (C) and 4 min (D), respectively. Scale bar, 25 μm.