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. 2019 Apr 12;9:5964. doi: 10.1038/s41598-019-42375-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunization of ApoE^−/− mice with collagen α1α2(IV)-PADRE results in an IgG1 response against collagen type IV. ApoE^−/− mice were immunized with collagen α1α2(IV)-PADRE peptides (25 or 50 µg), PADRE (8 or 16 µg) or PBS. Plasma from immunized mice were analyzed for IgG1 (dilution 1:5000) or IgG2c (dilution 1:500) for binding to en α1α2(IV) peptides (A,C) or PADRE (B,D) by ELISA. The specificity of IgG1 binding to collagen α1α2(IV) peptides was analyzed by addition of mouse collagen type IV protein at different concentrations (E). Kruskal-Wallis test followed by Dunn’s multiple comparisons post-test. Each dot represents one mouse and the bar denotes median value. (E) Values are represented as means of triplicates ± SD. **p < 0.005, ***p < 0.0001.