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. 2019 Apr 12;9:5964. doi: 10.1038/s41598-019-42375-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of T cells in spleen of ApoE^−/− mice immunized with collagen α1α2(IV)-PADRE. Splenocytes from mice immunized with collagen α1α2(IV)-PADRE peptides, PADRE or PBS were analyzed with flow cytometry. Representative flow cytometry gating strategy for CD3+CD4+, CD3+CD8+ and CD25+ FoxP3+. (A) Percentage of CD25+FoxP3+ (Treg) out of CD3+CD4+, (B) CD3+CD8+, (C) and CD3+CD4+cells, (D) for each treatment. ANOVA followed by Sidak’s multiple comparisons post-test with the bar denoting mean value. Each dot represents one mouse. *p < 0.05.