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. 2019 Apr 12;9:5964. doi: 10.1038/s41598-019-42375-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T cell related cytokines released by splenocytes from ApoE^−/− immunized with collagen α1α2(IV)-PADRE. Splenocytes from mice immunized with collagen α1α2(IV)-PADRE peptides, PADRE or PBS were stimulated with anti-CD3/CD28 beads and IFN-g (A), IL-12 (B), IL-4 (C), IL-5 (D), IL-10 (E), IL-13 (F), IL-17A (G) and IL-6 (H) were analyzed. ANOVA followed by Sidak’s multiple comparisons post-test where the bar denotes mean for normally distributed variables, or Kruskal-Wallis test followed by Dunn’s multiple comparisons post-test where the bar shows median value for non-normally distributed variables. Each dot represents one mouse. *p < 0.05, **p < 0.005, ***p < 0.0001.