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. 2019 Apr 12;9:6005. doi: 10.1038/s41598-019-42228-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GB total polyphenol content of GB is 59.91 ± 2.17 mg/L gallic acid equivalents (GAE). (a) Correlation between total polyphenolic content and the absorbance (A_735nm). The orange square corresponds to GB, 59.91 ± 2.17 mg/L GAE value, whereas purple squares to gallic acid working standards used as reference reagent. Linearity of calibration curve with correlation coefficient (R-squared, R² = 0.9919) and linear equation are included in the graph (y = 0.0058 × −0.039). (b) The equation used to calculate the GAE value. Each data point represents the mean of four replicas. For each data point, the standard deviations of absorbance (A₇₃₅ nm) were consistent with <0.072 (data not shown).